Treg细胞与Th17细胞失衡参与了不明原因复发性流产的发病

作者：Zhu L,et al.

翻译：刘湘源

摘要：调节性T细胞（Treg）和T辅助17细胞（Th17）是CD4阳性T细胞的两个亚型，在免疫反应中相互拮抗。最近的研究发现，这两类细胞的失调参与了不明原因复发性自然流产的发病。该研究的目的是，Treg/Th17平衡失调是否促进了不明原因流产发病及其影响因素。我们的研究发现，与健康组相比，不明原因复发流产组患者的Treg细胞比例及Foxp3-mRNA的表达均明显降低。但Th17细胞比例和ROR-γt- mRNA的表达明显增高，与Treg呈负相关。与正常对照组相比，不明原因复发性流产患者的Treg/Th17和 Foxp3/ROR-γt比值均降低，血清白介素-6和IL-17A明显增高，而IL-10明显降低，白介素-6水平与Th17,ROR-γt成正相关，而与Treg/Th17及Foxp3成负相关。该研究提示，Treg与Th17细胞之间的失衡可能参与了不明原因复发流产的发病，并与血清白介素6水平增高相关。

附原文：Abstract Regulatory T cells (Treg) and T helper 17 cells (Th17) are 2 distinct subsets of CD4+ T cells, which are mutually antagonistic in the immune response. Recently, dysregulation of these 2 cell subsets have been described in the pathogenesis of unexplained recurrentspontaneous abortion (URSA). The purpose of this study was to investigate whether the Treg/Th17 balance was perturbed in URSA patients and to explore contributing factors. We found that the proportion of Treg cells and expression of forkhead box P3 (Foxp3) messenger RNA (mRNA) were significantly lower in URSA patients than in healthy controls. However, the proportion of Th17 cells and expression of retinoid-related orphan nuclear

receptor-γt (ROR-γt) mRNA were higher in URSA patients than in controls, revealing inverse correlation with Treg. The ratio of Treg/Th17 and Foxp3/ROR-γt decreased in patients with URSA compared to healthy controls. The serum levels of interleukin (IL)-6 and IL-17A were significantly higher, whereas IL-10 was lower in URSA patients compared with controls, and the level of IL-6 showed a positive correlation with Th17, ROR-γt and inverse correlation with Treg, Foxp3. The present study indicated that an imbalance between Treg and Th17 cells might be implicated in the pathogenesis of URSA and this seems to relate to elevation in serum IL-6 level. 引自：Zhu L, Chen H, Liu M, et al.Treg/Th17 Cell Imbalance and IL-6 Profile in Patients With Unexplained Recurrent Spontaneous Abortion. Reprod Sci. 2016 Oct