NKT细胞功能水平较高提示多关节型幼年特发性关节炎对依那西普的疗效差

作者：Zhou J

翻译：刘佩玲 校正：于若寒

摘要：幼年特发性关节炎（JIA）具有三种临床表现广泛差异的主要发病类型。我们评估不同JIA亚型患者的自然杀伤T细胞（NKT）的功能，并发现与健康对照组相比，全身型患者NKT细胞计数、穿孔素及颗粒酶B表达减低，然而少关节型和多关节型患者表达高水平穿孔素及颗粒酶B。滑液中有更多NKT细胞，比外周血细胞有更高水平穿孔素、颗粒酶B和肿瘤坏死因子α（TNF-α）。与对依那西普治疗无效的患者相比，对其有疗效的多关节型患者有较低水平NKT细胞、细胞内穿孔素、颗粒酶B和TNF-α平均荧光强度。依那西普治疗可以降低治疗反应组NKT细胞的颗粒酶B和穿孔素，干扰素γ（IFN-γ）及 TNF-α的表达。因此，高NKT细胞功能可能表明多关节型患者对依那西普治疗反应欠佳。

附原文：Abstract：Juvenile idiopathic arthritis (JIA) has three major onset types with widely varying clinical features. We assessed the natural killer T (NKT) cell function in patients with different JIA subtypes, and found systemic patients exhibited lower NKT cell counts, perforin and granzyme B expression, while the pauciarticular and polyarticular patients displayed higher perforin and granzyme B expression as compared with the controls. The synovial fluid had more NKT cells with higher levels of perforin, granzyme B, and tumour necrosis factor (TNF)-α than peripheral cells. The polyarticular patients that responded to etanercept had lower NKT cell counts, intracellular perforin, granzyme B and the mean fluorescence intensity of TNF-α than the patients that did not respond. Treatment with etanercept reduced the granzyme B and perforin, interferon (IFN)-γ and TNF-α expression in NKT cells

in the responsive group. Therefore, a higher NKT cell function may indicate a decreased response to etanercept in polyarticular patients.

摘自：Zhou J, Ding Y, Zhang Y et al. CD3+CD56+ natural killer T cell activity in children with different forms of juvenile idiopathic arthritis and the influence of etanercept treatment on polyarticular subgroup. Clin Immunol. 2016 Dec 24;176:1-11. doi: 10.1016/j.clim.2016.12.001. [Epub ahead of print].